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Heparanase 1 polyclonal antibody

货号: BS80027

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS80027

Product Name :Heparanase 1 polyclonal antibody

Swiss-Prot :Q9Y251

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200

Background :Heparan sulfate proteoglycans are major components of the basement membrane and extracellular matrix. The protein encoded by this gene is an enzyme that cleaves heparan sulfate proteoglycans to permit cell movement through remodeling of the extracellular matrix. In addition, this cleavage can release bioactive molecules from the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :50kDa/65kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :HPSE;HPA;HPA1;HPR1;HPSE1;HSE1;heparanase

Immunogen :Recombinant fusion protein of human Heparanase1.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cells, using Heparanase 1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.

Immunofluorescence analysis of HeLa cells using Heparanase 1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.