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[KO Validated] HSP27/HSPB1 polyclonal antibody

货号: BS80009

价格：￥ 1540

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS80009

Product Name :[KO Validated] HSP27/HSPB1 polyclonal antibody

Swiss-Prot :P04792

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN).

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :27KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :HSPB1;CMT2F;HEL-S-102;HMN2B;HS.76067;HSP27;HSP28;Hsp25;SRP27

Immunogen :Recombinant fusion protein of human HSP27/HSP27/HSPB1(NP_001531.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts from normal and HSP27/HSP27/HSPB1 knockout HeLa cells, using HSP27/HSP27/HSPB1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 5s.

Western blot analysis of extracts of HeLa cells, using HSP27/HSP27/HSPB1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 60s.