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WT1 polyclonal antibody

货号: BS80004

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS80004

Product Name :WT1 polyclonal antibody

Swiss-Prot :P19544

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :49-60kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :WT1;AWT1;EWS-WT1;GUD;NPHS4;WAGR;WIT-2;WT33

Immunogen :Recombinant fusion protein of human WT1(P19544).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using WT1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.

Confocal immunofluorescence analysis of Hela cells using WT1 Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining.

Confocal immunofluorescence analysis of U-2 OS cells using WT1 Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of OVCAR-3 cells using WT1 Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.