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NLRP1 polyclonal antibody

货号: BS79994

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS79994

Product Name :NLRP1 polyclonal antibody

Swiss-Prot :Q9C000

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200

Background :This gene encodes a member of the Ced-4 family of apoptosis proteins. Ced-family members contain a caspase recruitment domain (CARD) and are known to be key mediators of programmed cell death. The encoded protein contains a distinct N-terminal pyrin-like motif, which is possibly involved in protein-protein interactions. This protein interacts strongly with caspase 2 and weakly with caspase 9. Overexpression of this gene was demonstrated to induce apoptosis in cells. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the biological validity of some variants has not been determined.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :160KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :NLRP1;AIADK;CARD7;CIDED;CLR17.1;DEFCAP;DEFCAP-L/S;MSPC;NAC;NALP1;PP1044;SLEV1;VAMAS1

Immunogen :Recombinant fusion protein of human NLRP1(NP_127497.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of K-562 cells, using NLRP1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit .
Exposure time: 180s.

Immunohistochemistry of paraffin-embedded human colon using NLRP1 Rabbit pAb at dilution of 1:50 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded human tonsil using NLRP1 Rabbit pAb at dilution of 1:50 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunofluorescence analysis of K-562 cells using NLRP1 Rabbit pAb at dilution of 1:50 . Blue: DAPI for nuclear staining.