产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

Sin3A polyclonal antibody

货号: BS79984

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS79984

Product Name :Sin3A polyclonal antibody

Swiss-Prot :Q96ST3

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC, IP, ChIP

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200|IP,1:50 - 1:100|ChIP,1:20 - 1:100

Background :The protein encoded by this gene is a transcriptional regulatory protein. It contains paired amphipathic helix (PAH) domains, which are important for protein-protein interactions and may mediate repression by the Mad-Max complex.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :160KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :SIN3A;WITKOS

Immunogen :A synthetic peptide of human Sin3A(NP_056292.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using Sin3A antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.

Immunofluorescence analysis of U2OS cells using Sin3A antibody . Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 300ug extracts of Jurkat cells using 3ug Sin3A antibody . Western blot was performed from the immunoprecipitate using Sin3A antibody at a dilution of 1:1000.