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Calnexin polyclonal antibody

货号: BS79966

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS79966

Product Name :Calnexin polyclonal antibody

Swiss-Prot :P27824

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC, IP

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200|IP,1:50 - 1:200

Background :This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :90KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CNX;IP90;P90;Calnexin;CANX;calnexin

Immunogen :A synthetic peptide of human Calnexin(NP_001737.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using Calnexin antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.

Immunofluorescence analysis of PC-12 cells using Calnexin Rabbit pAb at dilution of 1:25 . Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 300ug extracts of NIH/3T3 cells using 3ug Calnexin antibody . Western blot was performed from the immunoprecipitate using Calnexin antibody at a dilution of 1:500.