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MYH2 polyclonal antibody

货号: BS79957

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS79957

Product Name :MYH2 polyclonal antibody

Swiss-Prot :Q9UKX2

Host :Rabbit

Reactivity :Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:200 - 1:2000|IF/ICC,1:50 - 1:200

Background :Myosins are actin-based motor proteins that function in the generation of mechanical force in eukaryotic cells. Muscle myosins are heterohexamers composed of 2 myosin heavy chains and 2 pairs of nonidentical myosin light chains. This gene encodes a member of the class II or conventional myosin heavy chains, and functions in skeletal muscle contraction. This gene is found in a cluster of myosin heavy chain genes on chromosome 17. A mutation in this gene results in inclusion body myopathy-3. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :250KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :MYH2;IBM3;MYH2A;MYHSA2;MYHas8;MYPOP;MyHC-2A;MyHC-IIa;myosin-2

Immunogen :A synthetic peptide of human MYH2(NP_001093582.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of Rat skeletal muscle, using MYH2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.

Western blot analysis of extracts of Mouse skeletal muscle, using MYH2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 10s.

Immunofluorescence analysis of mouse skeletal muscle cells using MYH2 Rabbit pAb at dilution of 1:50 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of rat skeletal muscle cells using MYH2 Rabbit pAb at dilution of 1:50 . Blue: DAPI for nuclear staining.