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ZEB1 polyclonal antibody

货号: BS79939

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS79939

Product Name :ZEB1 polyclonal antibody

Swiss-Prot :P37275

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:1000|IHC,1:50 - 1:200|IF/ICC,1:100 - 1:200

Background :This gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have been associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :200KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :ZEB1;AREB6;BZP;DELTAEF1;FECD6;NIL2A;PPCD3;TCF8;ZFHEP;ZFHX1A

Immunogen :Recombinant fusion protein of human ZEB1(NP_110378.3).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of Jurkat cells, using ZEB1 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 3s.

Immunohistochemistry of paraffin-embedded human gastric cancer using ZEB1 antibody at dilution of 1:200 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.