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PTCH1 polyclonal antibody

货号: BS79924

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS79924

Product Name :PTCH1 polyclonal antibody

Swiss-Prot :Q13635

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :This gene encodes a member of the patched gene family. The encoded protein is the receptor for sonic hedgehog, a secreted molecule implicated in the formation of embryonic structures and in tumorigenesis, as well as the desert hedgehog and indian hedgehog proteins. This gene functions as a tumor suppressor. Mutations of this gene have been associated with basal cell nevus syndrome, esophageal squamous cell carcinoma, trichoepitheliomas, transitional cell carcinomas of the bladder, as well as holoprosencephaly. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences and biological validity cannot be determined currently.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :160kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :PTCH1;BCNS;HPE7;NBCCS;PTC;PTC1;PTCH;PTCH11;patched 1

Immunogen :Recombinant fusion protein of human PTCH1(NP_000255.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of Rat skeletal muscle, using PTCH1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 10s.

Immunofluorescence analysis of mouse embryos cells using PTCH1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.