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S100A10 polyclonal antibody

货号: BS79910

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS79910

Product Name :S100A10 polyclonal antibody

Swiss-Prot :P60903

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:25 - 1:200|IF/ICC,1:25 - 1:200

Background :The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in exocytosis and endocytosis.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :11KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :S100A10;42C;ANX2L;ANX2LG;CAL1L;CLP11;Ca[1];GP11;P11;p10

Immunogen :Recombinant fusion protein of human S100A10(NP_002957.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using S100A10 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit .
Exposure time: 180s.

Immunohistochemistry of paraffin-embedded Human esophagus using S100A10 antibody at dilution of 1:25 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunofluorescence analysis of PC-12 using S100A10 antibody at dilution of 1:25 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of A-431 using S100A10 antibody at dilution of 1:25 . Blue: DAPI for nuclear staining.