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Histone H3.3 polyclonal antibody

货号: BS79854

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS79854

Product Name :Histone H3.3 polyclonal antibody

Swiss-Prot :P84243

Host :Rabbit

Reactivity :Human, Mouse, Rat, Other (Wide Range)

Applications :WB

Application_all :WB,1:500 - 1:2000

Background :Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :16kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :H3F3A;H3.3A;H3F3;histone H3.3

Immunogen :Recombinant fusion protein of human H3F3A(NP_002098.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using Histone H3.3 antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.