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NR5A2 polyclonal antibody

货号: BS79852

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS79852

Product Name :NR5A2 polyclonal antibody

Swiss-Prot :O00482

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200

Background :The protein encoded by this gene is a DNA-binding zinc finger transcription factor and is a member of the fushi tarazu factor-1 subfamily of orphan nuclear receptors. The encoded protein is involved in the expression of genes for hepatitis B virus and cholesterol biosynthesis, and may be an important regulator of embryonic development.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :61KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :NR5A2;B1F;B1F2;CPF;FTF;FTZ-F1;FTZ-F1beta;LRH-1;LRH1;hB1F-2

Immunogen :Recombinant fusion protein of human NR5A2(NP_995582.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of various lysates, using NR5A2 antibody at 1:2680 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 20s.

Immunohistochemistry of paraffin-embedded rat pancreas using NR5A2 Rabbit pAb at dilution of 1:100 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded mouse pancreas using NR5A2 Rabbit pAb at dilution of 1:100 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunofluorescence analysis of rat liver cells using NR5A2 Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.