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Tyrosinase polyclonal antibody

货号: BS79841

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS79841

Product Name :Tyrosinase polyclonal antibody

Swiss-Prot :P14679

Host :Rabbit

Reactivity :Human, Rat

Applications :WB

Application_all :WB,1:500 - 1:2000

Background :The enzyme encoded by this gene catalyzes the first 2 steps, and at least 1 subsequent step, in the conversion of tyrosine to melanin. The enzyme has both tyrosine hydroxylase and dopa oxidase catalytic activities, and requires copper for function. Mutations in this gene result in oculocutaneous albinism, and nonpathologic polymorphisms result in skin pigmentation variation. The human genome contains a pseudogene similar to the 3' half of this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :80kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :TYR;ATN;CMM8;OCA1;OCA1A;OCAIA;SHEP3

Immunogen :Recombinant fusion protein of human Tyrosinase(NP_000363.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of rat skin, using Tyrosinase antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 10s.