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AKR1C3 polyclonal antibody

货号: BS79838

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :BS79838

Product Name :AKR1C3 polyclonal antibody

Swiss-Prot :P42330

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200

Background :This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ), and the oxidation of 9alpha,11beta-PGF2 to PGD2. It may play an important role in the pathogenesis of allergic diseases such as asthma, and may also have a role in controlling cell growth and/or differentiation. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. Three transcript variants encoding different isoforms have been found for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :37KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :AKR1C3;DD3;DDX;HA1753;HAKRB;HAKRe;HSD17B5;PGFS;hluPGFS

Immunogen :Recombinant fusion protein of human AKR1C3(NP_003730.4).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of various lysates, using AKR1C3 antibody at 1:2510 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.

Immunohistochemistry of paraffin-embedded rat heart using AKR1C3 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.