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[KO Validated] Galectin 3/LGALS3 polyclonal antibody

货号: BS79830

价格：￥ 1540

货期：现货（1-2个工作日）

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No. :BS79830

Product Name :[KO Validated] Galectin 3/LGALS3 polyclonal antibody

Swiss-Prot :P17931

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :28KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :LGALS3;CBP35;GAL3;GALBP;GALIG;L31;LGALS2;MAC2

Immunogen :Recombinant fusion protein of human Galectin 3/Galectin 3/LGALS3(NP_002297.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts from normal and Galectin 3/Galectin 3/LGALS3 knockout HeLa cells, using Galectin 3/Galectin 3/LGALS3 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.

Immunofluorescence analysis of C6 cells using Galectin 3/Galectin 3/LGALS3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of L-929 cells using Galectin 3/Galectin 3/LGALS3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using Galectin 3/Galectin 3/LGALS3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.