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ADH5/GSNOR polyclonal antibody

货号: BS79822

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS79822

Product Name :ADH5/GSNOR polyclonal antibody

Swiss-Prot :P11766

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :This gene encodes a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. The encoded protein forms a homodimer. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long-chain primary alcohols and for oxidation of S-hydroxymethyl-glutathione, a spontaneous adduct between formaldehyde and glutathione. This enzyme is an important component of cellular metabolism for the elimination of formaldehyde, a potent irritant and sensitizing agent that causes lacrymation, rhinitis, pharyngitis, and contact dermatitis. The human genome contains several non-transcribed pseudogenes related to this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :40KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :ADH5;ADH-3;ADHX;FALDH;FDH;GSH-FDH;GSNOR;HEL-S-60p

Immunogen :Recombinant fusion protein of human ADH5/GSNOR(NP_000662.3).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using ADH5/GSNOR antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.

Immunofluorescence analysis of HeLa cells using ADH5/GSNOR Rabbit pAb at dilution of 1:50 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HepG2 cells using ADH5/GSNOR Rabbit pAb at dilution of 1:50 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using ADH5/GSNOR Rabbit pAb at dilution of 1:50 . Blue: DAPI for nuclear staining.