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Rig-I/DDX58 polyclonal antibody

货号: BS79815

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS79815

Product Name :Rig-I/DDX58 polyclonal antibody

Swiss-Prot :O95786

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of immune response.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :102-110kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :DDX58;RIG-I;RIGI;RLR-1;SGMRT2

Immunogen :Recombinant fusion protein of human Rig-I/DDX58(NP_055129.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using Rig-I/DDX58 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit .
Exposure time: 90s.

Immunofluorescence analysis of H9C2 cells using Rig-I/DDX58 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of L929 cells using Rig-I/DDX58 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U20S cells using Rig-I/DDX58 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.