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CREBBP polyclonal antibody

货号: BS79812

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS79812

Product Name :CREBBP polyclonal antibody

Swiss-Prot :Q92793

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB,IHC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200

Background :This gene is ubiquitously expressed and is involved in the transcriptional coactivation of many different transcription factors. First isolated as a nuclear protein that binds to cAMP-response element binding protein (CREB), this gene is now known to play critical roles in embryonic development, growth control, and homeostasis by coupling chromatin remodeling to transcription factor recognition. The protein encoded by this gene has intrinsic histone acetyltransferase activity and also acts as a scaffold to stabilize additional protein interactions with the transcription complex. This protein acetylates both histone and non-histone proteins. This protein shares regions of very high sequence similarity with protein p300 in its bromodomain, cysteine-histidine-rich regions, and histone acetyltransferase domain. Mutations in this gene cause Rubinstein-Taybi syndrome (RTS). Chromosomal translocations involving this gene have been associated with acute myeloid leukemia. Alternative splicing results in multiple transcript variants encoding different isoforms.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :300KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CREBBP;CBP;KAT3A;RSTS;RSTS1

Immunogen :A synthetic peptide of human CREBBP(NP_004371.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of NIH/3T3 cells, using CREBBP antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit .
Exposure time: 180s.

Immunohistochemistry of paraffin-embedded human colon carcinoma using CREBBP Rabbit pAb at dilution of 1:250 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded human esophageal cancer using CREBBP Rabbit pAb at dilution of 1:250 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.