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Tyrosine Hydroxylase polyclonal antibody

货号: BS79775

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS79775

Product Name :Tyrosine Hydroxylase polyclonal antibody

Swiss-Prot :P07101

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200

Background :The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :60KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :DYT14;DYT5b;TYH;TH

Immunogen :Recombinant fusion protein of human Tyrosine Hydroxylase(NP_954986.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of various lysates, using Tyrosine Hydroxylase antibody at 1:2630 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 10s.

Immunohistochemistry of paraffin-embedded rat adrenal gland using Tyrosine Hydroxylase Rabbit pAb at dilution of 1:25 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunofluorescence analysis of Neuro-2α cells using Tyrosine Hydroxylase Rabbit pAb at dilution of 1:150 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of SH-SY5Y cells using Tyrosine Hydroxylase Rabbit pAb at dilution of 1:150 . Blue: DAPI for nuclear staining.