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[KO Validated] IKKγ polyclonal antibody

货号: BS79757

价格：￥ 1540

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS79757

Product Name :[KO Validated] IKKγ polyclonal antibody

Swiss-Prot :Q9Y6K9

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB, IP

Application_all :WB,1:500 - 1:2000|IP,1:50 - 1:100

Background :This gene encodes the regulatory subunit of the inhibitor of kappaB kinase (IKK) complex, which activates NF-kappaB resulting in activation of genes involved in inflammation, immunity, cell survival, and other pathways. Mutations in this gene result in incontinentia pigmenti, hypohidrotic ectodermal dysplasia, and several other types of immunodeficiencies. A pseudogene highly similar to this locus is located in an adjacent region of the X chromosome.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :48kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :IKBKG;AMCBX1;FIP-3;FIP3;Fip3p;IKK-gamma;IKKAP1;IKKG;IMD33;IP;IP1;IP2;IPD2;NEMO;ZC2HC9

Immunogen :Recombinant fusion protein of human IKKγ(NP_003630.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using IKKγ Antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Western blot analysis of extracts from normal and IKKγ knockout 293T cells, using IKKγ antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 5s.

Immunoprecipitation analysis of 200ug extracts of A549 cells using 3ug IKKγ antibody . Western blot was performed from the immunoprecipitate using IKKγ antibody at a dilution of 1:1000.