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Aggrecan polyclonal antibody

货号: BS79720

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS79720

Product Name :Aggrecan polyclonal antibody

Swiss-Prot :P16112

Host :Rabbit

Reactivity :Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :This gene is a member of the aggrecan/versican proteoglycan family. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Multiple alternatively spliced transcript variants that encode different protein isoforms have been observed in this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :230kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :ACAN;AGC1;AGCAN;CSPG1;CSPGCP;MSK16;SEDK;aggrecan

Immunogen :Recombinant fusion protein of human Aggrecan(NP_037359.3).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of mouse brain, using Aggrecan antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit .
Exposure time: 90s.

Immunofluorescence analysis of Mouse cartilage cells using Aggrecan Rabbit pAb at dilution of 1:50 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of Rat cartilage cells using Aggrecan antibody at dilution of 1:100 .Blue: DAPI for nuclear staining.