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LC3B polyclonal antibody

货号: BS79705

价格：￥ 1680

货期：现货（1-2个工作日）

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No. :BS79705

Product Name :LC3B polyclonal antibody

Swiss-Prot :Q9GZQ8

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC, IP

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:100|IF/ICC,1:50 - 1:100|IP,1:20 - 1:50

Background :The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :20kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :LC3B;ATG8F;MAP1LC3B-a;MAP1A/1BLC3;MAP1LC3B;LC3A/LC3B

Immunogen :A synthetic peptide of human LC3B(NP_073729.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of 293 cells, using LC3B antibody .
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Western blot analysis of extracts of various cell lines, using LC3B antibody at 1:1000 dilution.Hela cells were treated by Chloroquine at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit .
Exposure time: 60s.

Immunohistochemistry of paraffin-embedded rat brain using LC3B Rabbit pAb at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded human colon using LC3B Rabbit pAb at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.