Western blot analysis of extracts of 293T cells, using LC3B antibody at 1:1000 dilution.293T cells were treated by Chloroquine at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit .
Exposure time: 180s.
Western blot analysis of extracts from normal and LC3B knockout 293T cells, using LC3B antibody at 1:1000 dilution.293T cells were treated by Chloroquine at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit .
Exposure time: 180s.
Western blot analysis of extracts from normal and LC3B knockout 293T cells, using LC3B antibody at 1:1000 dilution.293T cells were treated by Chloroquine at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human breast cancer using [KO Validated] LC3B Rabbit pAb at dilution of 1:100 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
