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GPER1 polyclonal antibody

货号: BS79583

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS79583

Product Name :GPER1 polyclonal antibody

Swiss-Prot :Q99527

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :This gene is a member of the G-protein coupled receptor 1 family and encodes a multi-pass membrane protein that localizes to the endoplasmic reticulum. The protein binds estrogen, resulting in intracellular calcium mobilization and synthesis of phosphatidylinositol 3,4,5-trisphosphate in the nucleus. This protein therefore plays a role in the rapid nongenomic signaling events widely observed following stimulation of cells and tissues with estrogen. Alternate transcriptional splice variants which encode the same protein have been characterized.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :52KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :GPER1;CEPR;CMKRL2;DRY12;FEG-1;GPCR-Br;GPER;GPR30;LERGU;LERGU2;LyGPR;mER;GPER1

Immunogen :Recombinant fusion protein of human GPER1(Q99527).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of Mouse heart cells, using GPER1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 5s.

Immunofluorescence analysis of NIH/3T3 cells using GPER1 antibody at dilution of 1:100 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U20S cells using GPER1 antibody at dilution of 1:100 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of C6 cells using GPER1 antibody at dilution of 1:100 . Blue: DAPI for nuclear staining.