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eIF2α polyclonal antibody

货号: BS79569

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS79569

Product Name :eIF2α polyclonal antibody

Swiss-Prot :P05198

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB

Application_all :WB,1:500 - 1:1000

Background :The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010]

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :38KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :EIF-2;EIF-2A;EIF-2alpha;EIF2;EIF2A;EIF2S1

Immunogen :Recombinant fusion protein of human eIF2α(NP_004085.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of various lysates, using eIF2α antibody at 1:420 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 15s.

A neuroprotective role of Ufmylation through Atg9 in the aging brain of Drosophila

JournalCELLULAR AND MOLECULAR LIFE SCIENCES

ApplicationWB

IF9.207

SpeciesDrosophila

PMID37086384