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STAT6 polyclonal antibody

货号: BS79568

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS79568

Product Name :STAT6 polyclonal antibody

Swiss-Prot :P42226

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein plays a central role in exerting IL4 mediated biological responses. It is found to induce the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL4. Knockout studies in mice suggested the roles of this gene in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins. Alternative splicing results in multiple transcript variants.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :110KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :D12S1644;IL-4-STAT;STAT6B;STAT6C;STAT6

Immunogen :A synthetic peptide of human STAT6(NP_003144.3).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using STAT6 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 10s.

Immunofluorescence analysis of HeLa cells using STAT6 Rabbit pAb at dilution of 1:200 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using STAT6 Rabbit pAb at dilution of 1:200 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using STAT6 Rabbit pAb at dilution of 1:200 . Blue: DAPI for nuclear staining.