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Bak polyclonal antibody

货号: BS79525

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS79525

Product Name :Bak polyclonal antibody

Swiss-Prot :Q16611

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC, IP

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200|IP,1:50 - 1:200

Background :The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :25KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :BAK1;BAK;BAK-LIKE;BCL2L7

Immunogen :A synthetic peptide of human Bak(NP_001179.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of THP-1 cells, using Bak antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.

Immunofluorescence analysis of C6 cells using Bak Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using Bak Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 300ug extracts of THP-1 cells using 3ug Bak antibody . Western blot was performed from the immunoprecipitate using Bak antibody at a dilution of 1:1000.