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Phospho-PTEN-S380/T382/383 polyclonal antibody

货号: BS79503

价格：￥ 1680

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS79503

Product Name :Phospho-PTEN-S380/T382/383 polyclonal antibody

Swiss-Prot :P60484

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :IHC

Application_all :IHC,1:50 - 1:200

Background :This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Phosphorylated

BiowMW :Refer to figures

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :10q23del;BZS;CWS1;DEC;GLM2;MHAM;MMAC1;PTEN1;TEP1;PTEN;PTENbeta

Immunogen :A synthetic phosphorylated peptide around S380 & T382 & T383 of human PTEN(NP_000305.3).

Conjugate :Unconjugated

Modification :Phosphorylated

Immunohistochemistry of paraffin-embedded human colon carcinoma using Phospho-PTEN-S380/T382/383 Rabbit pAb at dilution of 1:200 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded mouse heart using Phospho-PTEN-S380/T382/383 Rabbit pAb at dilution of 1:200 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded rat lung using Phospho-PTEN-S380/T382/383 Rabbit pAb at dilution of 1:200 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.