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Phospho-p38 MAPK-T180/Y182 polyclonal antibody

货号: BS79490
价格 1680
货期 现货(1-2个工作日)
25ul 50ul 100ul 200ul
No. :BS79490
Product Name :Phospho-p38 MAPK-T180/Y182 polyclonal antibody
Swiss-Prot :Q15759/P53778/O15264/Q16539
Host :Rabbit
Reactivity :Mouse, Rat
Applications :WB
Application_all :WB,1:500 - 1:2000
Background :This gene encodes a member of a family of protein kinases that are involved in the integration of biochemical signals for a wide variety of cellular processes, including cell proliferation, differentiation, transcriptional regulation, and development. The encoded protein can be activated by proinflammatory cytokines and environmental stresses through phosphorylation by mitogen activated protein kinase kinases (MKKs). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]
Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Phosphorylated
BiowMW :43KDa
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :MAPK14;CSBP;CSBP1;CSBP2;CSPB1;EXIP;Mxi2;PRKM14;PRKM15;RK;SAPK2A;p38;p38ALPHA;p38 MAPK
Immunogen :A synthetic phosphorylated peptide around T180 & Y182 of human MAPK11/ MAPK14/MAPK12MAPK11(NP_002742.3).
Conjugate :Unconjugated
Modification :Phosphorylated
Western blot analysis of extracts of various cell lines, using Phospho-p38 MAPK-T180/Y182 antibody at 1:1000 dilution.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 60s.
Western blot analysis of extracts of various cell lines, using Phospho-p38 MAPK-T180/Y182 antibody at 1:1000 dilution.C6 cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.

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400-660-9091

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com