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Phospho-Bcl-2-S87 polyclonal antibody

货号: BS79474

价格：￥ 1680

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS79474

Product Name :Phospho-Bcl-2-S87 polyclonal antibody

Swiss-Prot :P10415

Host :Rabbit

Reactivity :Human, Rat

Applications :WB

Application_all :WB,1:500 - 1:2000

Background :This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Phosphorylated

BiowMW :28kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Bcl-2;PPP1R50;BCL2

Immunogen :A synthetic phosphorylated peptide around S87 of human Bcl-2(NP_000624.2).

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot analysis of extracts of C6 cells, using Phospho-Bcl-2-S87 antibody at 1:1000 dilution.C6 cells were treated by Paclitaxel at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit .
Exposure time: 10s.