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Phospho-C/EBPB-T235 polyclonal antibody

货号: BS79468

价格：￥ 1680

货期：现货（1-2个工作日）

25ul 50ul 100ul

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS79468

Product Name :Phospho-C/EBPB-T235 polyclonal antibody

Swiss-Prot :P17676

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB

Application_all :WB,1:500 - 1:2000

Background :This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain. The encoded protein functions as a homodimer but can also form heterodimers with CCAAT/enhancer-binding proteins alpha, delta, and gamma. Activity of this protein is important in the regulation of genes involved in immune and inflammatory responses, among other processes. The use of alternative in-frame AUG start codons results in multiple protein isoforms, each with distinct biological functions.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Phosphorylated

BiowMW :38KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :C/EBP-beta;IL6DBP;NF-IL6;TCF5;CEBP beta;CEBPB

Immunogen :A synthetic phosphorylated peptide around T235 of human C/EBPB(NP_005185.2).

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot analysis of extracts of NIH/3T3, using Phospho-C/EBPB-T235 antibody at 1:1000 dilution.NIH/3T3 cells were treated by CIP at 37℃ for 1 hour.NIH/3T3 cells were treated by Serum-starvation overnight at 37℃.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.