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Phospho-(Ser/Thr) ATM/ATR Substrate polyclonal antibody

货号: BS79460

价格：￥ 1680

货期：现货（1-2个工作日）

25ul 50ul 100ul

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS79460

Product Name :Phospho-(Ser/Thr) ATM/ATR Substrate polyclonal antibody

Swiss-Prot :P08631

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB

Application_all :WB,1:500 - 1:2000

Background :The functionally related ATM (ataxia telangiectasia-mutated) and ATR (ATM-Rad3-related) protein kinases are critical regulators of DNA damage responses in mammalian cells. ATM and ATR share highly overlapping substrate specificities and show a strong preference for the phosphorylation of Serine (S) or Threonine (T) residues followed by Gln. It also called SQ or TQ consensus sites.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Phosphorylated

BiowMW :38-68KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :HCK;JTK9;p59Hck;p61Hck

Immunogen :A synthetic peptide corresponding to a sequence containing phosphorylated S &T.

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot analysis of extracts of various cell lines, using Phospho- ATM/ATR Substrate pAb at 1:1000 dilution.Jurkat and NIH/3T3 cells were treated by Calyculin A at 37℃ for 30 minutes after serum-starvation overnight.C6 cells were treated by ATP at 30℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 60s.