产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

Pan Phospho-Tyrosine polyclonal antibody

货号: BS79455

价格：￥ 1680

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS79455

Product Name :Pan Phospho-Tyrosine polyclonal antibody

Host :Rabbit

Reactivity :Human, Mouse, Rat, Other (Wide Range)

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :Tyrosine phosphorylation (pTyr), much of which occurred on localized multiple sites, initiates cellular signaling, governs cellular functions, and its dysregulation is implicated in many diseases, especially cancers. pTyr-specific sensing is of great significance for understanding disease states and developing targeted anticancer drugs.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Phosphorylated

BiowMW :20-70KDa

Note :For research use only, not for use in diagnostic procedure.

Immunogen :A synthetic peptide corresponding to a sequence containing phosphorylated S &T.

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot analysis of extracts of Jurkat cells, using Pan Phospho-Tyrosine pAb at 1:1000 dilution.Jurkat cells were treated by Pervanadate at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit .
Exposure time: 1s.

Immunofluorescence analysis of C6 cells using Pan Phospho-Tyrosine at dilution of 1:100. Blue: DAPI for nuclear staining.C6 cells were treated by 20% FBS at 37℃ for 20 minutes after serum-starvation overnight.

Immunofluorescence analysis of NIH/3T3 cells using Pan Phospho-Tyrosine at dilution of 1:100. Blue: DAPI for nuclear staining.NIH/3T3 cells were treated by 20% FBS at 37℃ for 20 minutes after serum-starvation overnight.