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Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 polyclonal antibody

货号: BS79452

价格：￥ 1680

货期：现货（1-2个工作日）

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No. :BS79452

Product Name :Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 polyclonal antibody

Swiss-Prot :Q12778/O43524

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200

Background :This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq, Jul 2008]

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Phosphorylated

BiowMW :65KDa/73KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :FoxO1/FoxO3a/FoxO4

Immunogen :A synthetic phosphorylated peptide around T24 of human FOXO1/FOXO3A/FOXO4FOXO1(NP_002006.2).

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot analysis of extracts of various cell lines, using Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 antibody at 1:500 dilution.Jurkat cells were treated by Calyculin A at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.

Immunohistochemistry of paraffin-embedded rat liver using Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 antibody at dilution of 1:200 .Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded human liver cancer using Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 antibody at dilution of 1:200 .Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded mouse kidney using Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 antibody at dilution of 1:200 .Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.