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Phospho-Histone H3-S28 polyclonal antibody

货号: BS79443

价格：￥ 1680

货期：现货（1-2个工作日）

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No. :BS79443

Product Name :Phospho-Histone H3-S28 polyclonal antibody

Swiss-Prot :Q16695

Host :Rabbit

Reactivity :Human, Mouse, Rat, Other (Wide Range)

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Phosphorylated

BiowMW :17kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :H3/A;H3C2;H3C3;H3C4;H3C6;H3C7;H3C8;H3FA;H3C10;H3C11;H3C12;HIST1H3A

Immunogen :A synthetic phosphorylated peptide around S28 of human Histone H3(NP_003520.1).

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot analysis of extracts of various cell lines, using Phospho-Histone H3-S28 antibody at 1:1000 dilution. Both HeLa cells and NIH/3T3 cells were treated by Calyculin A at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit .
Exposure time: 1s.

Immunofluorescence analysis of H9C2 cells using Phospho-Histone H3-S28 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using Phospho-Histone H3-S28 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS cells using Phospho-Histone H3-S28 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.