产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

Phospho-NF-kB p65/RelA-T434 polyclonal antibody

货号: BS79442

价格：￥ 1680

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS79442

Product Name :Phospho-NF-kB p65/RelA-T434 polyclonal antibody

Swiss-Prot :Q04206

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB

Application_all :WB,1:500 - 1:2000

Background :NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Phosphorylated

BiowMW :58kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :NFKB3;p65;NF-kB p65;RELA;CMCU

Immunogen :A synthetic phosphorylated peptide around T435 of human RelA(NP_068810.3).

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot analysis of extracts of NIH/3T3 cells, using Phospho-NF-kB p65/RelA-T434 pAb at 1:1000 dilution or RelA antibody .NIH/3T3 cells were treated by TNF-α at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit .
Exposure time: 5min.