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Phospho-eIF4E-S209 polyclonal antibody

货号: BS79422

价格：￥ 1680

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS79422

Product Name :Phospho-eIF4E-S209 polyclonal antibody

Swiss-Prot :P06730

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB

Application_all :WB,1:1000 - 1:2000

Background :The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Phosphorylated

BiowMW :25kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :AUTS19;CBP;EIF4E1;EIF4EL1;EIF4F;eIF-4E;EIF4E

Immunogen :A synthetic phosphorylated peptide around S209 of human eIF4E(NP_001959.1).

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot analysis of extracts of NIH/3T3 cells, using Phospho-eIF4E-S209 antibody at 1:500 dilution.NIH/3T3 cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit .
Exposure time: 3min.