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Phospho-Bcl-2-S70 polyclonal antibody

货号: BS79406

价格：￥ 1680

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS79406

Product Name :Phospho-Bcl-2-S70 polyclonal antibody

Swiss-Prot :P10415

Host :Rabbit

Reactivity :Human, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Phosphorylated

BiowMW :28kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Bcl-2;PPP1R50;BCL2

Immunogen :A synthetic phosphorylated peptide around S70 of human Bcl-2(NP_000624.2).

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot analysis of extracts of Jurkat and 293 cells, using Phospho-Bcl2 -S70 antibody at 1:1000 dilution. Jurkat cells were treated by serum-starvation overnight. 293 cells were treated by UV for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.

Immunofluorescence analysis of HeLa cells using BCL2 antibody at dilution of 1:100 . Blue: DAPI for nuclear staining.