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Phospho-p70 S6 Kinase 1-T389 polyclonal antibody

货号: BS79403

价格：￥ 1680

货期：现货（1-2个工作日）

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No. :BS79403

Product Name :Phospho-p70 S6 Kinase 1-T389 polyclonal antibody

Swiss-Prot :P23443

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Phosphorylated

BiowMW :70KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :PS6K;S6K;S6K-beta-1;S6K1;STK14A;p70 S6KA;p70(S6K)-alpha;p70-S6K;p70-alpha;P70 S6K;RPS6KB1;p70S6KA

Immunogen :A synthetic phosphorylated peptide around T389 of human P70 S6K(NP_003152.1).

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot analysis of NIH/3T3, using Phospho-p70 S6 Kinase 1-T389 antibody at 1:500 dilution.NIH/3T3 cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.

Immunofluorescence analysis of C6 cells using Phospho-p70 S6 Kinase 1-T389 antibody at dilution of 1:100. C6 cells were treated by Serum-starvation overnight at 37℃.

Immunofluorescence analysis of HeLa cells using Phospho-p70 S6 Kinase 1-T389 antibody at dilution of 1:100. HeLa cells were treated by Serum-starvation overnight at 37℃.