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Phospho-FAK-Y576/577 polyclonal antibody

货号: BS79395
价格 1680
货期 现货(1-2个工作日)
25ul 50ul 100ul 200ul
No. :BS79395
Product Name :Phospho-FAK-Y576/577 polyclonal antibody
Swiss-Prot :Q05397
Host :Rabbit
Reactivity :Human
Applications :WB
Application_all :WB,1:500 - 1:2000
Background :This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene.
Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Phosphorylated
BiowMW :130kDa
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :FADK;FAK;FAK1;FRNK;PPP1R71;p125FAK;pp125FAK;PTK2
Immunogen :A synthetic phosphorylated peptide around Y576 & Y577 of human PTK2(NP_722560.1).
Conjugate :Unconjugated
Modification :Phosphorylated
Western blot analysis of extracts of Jurkat and Raji cells, using Phospho-FAK-Y576/577 antibody at 1:1000 dilution. Jurkat cells were treated by CD-3 for 2 minutes after serum-starvation overnight or treated by PMA/TPA for 10 minutes.Raji cells were treated by PMA/TPA for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit .
Exposure time: 90s.

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400-660-9091

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com