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Phospho-AURKA-T288 polyclonal antibody

货号: BS79389

价格：￥ 1680

货期：现货（1-2个工作日）

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No. :BS79389

Product Name :Phospho-AURKA-T288 polyclonal antibody

Swiss-Prot :O14965

Host :Rabbit

Reactivity :Human

Applications :WB

Application_all :WB,1:500 - 1:2000

Background :The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Phosphorylated

BiowMW :48kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :AURKA;AIK;ARK1;AURA;BTAK;PPP1R47;STK15;STK6;STK7

Immunogen :A synthetic phosphorylated peptide around T288 of human AURKA(NP_003591.2).

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot analysis of extracts of HeLa and HT-29 cells, using Phospho-AURKA-T288 antibody at 1:1000 dilution. HeLa cells were treated by Hydroxyurea for 20 hours.HeLa cells were treated by Nocodazole for 20 hours.HT-29 cells were treated by Hydroxyurea for 20 hours or treated by Nocodazole for 16 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit .
Exposure time: 90s.