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Phospho-Src-S17 polyclonal antibody

货号: BS79388
价格 1680
货期 现货(1-2个工作日)
25ul 50ul 100ul 200ul
No. :BS79388
Product Name :Phospho-Src-S17 polyclonal antibody
Swiss-Prot :P12931
Host :Rabbit
Reactivity :Human, Mouse, Rat
Applications :WB
Application_all :WB,1:500 - 1:2000
Background :This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Phosphorylated
BiowMW :60KDa
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :ASV;SRC1;THC6;c-SRC;p60-Src;SRC
Immunogen :A synthetic phosphorylated peptide around S17 of human Phospho-Src-S17(NP_005408.1).
Conjugate :Unconjugated
Modification :Phosphorylated
Western blot analysis of extracts of 293T cells, using Phospho-Src-S17 antibody at 1:1000 dilution.293T cells were treated by CIP at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.

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400-660-9091

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com