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Phospho-ZAP70-Y493 polyclonal antibody

货号: BS79376

价格：￥ 1680

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :BS79376

Product Name :Phospho-ZAP70-Y493 polyclonal antibody

Swiss-Prot :P43403

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:2000 - 1:5000|IHC,1:50 - 1:100|IF/ICC,1:50 - 1:200

Background :This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Phosphorylated

BiowMW :70KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :ZAP70;ADMIO2;IMD48;SRK;STCD;STD;TZK;ZAP-70

Immunogen :A synthetic phosphorylated peptide around Y493 of human ZAP70(NP_001070.2).

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot analysis of extracts of Jurkat cells, using Phospho-ZAP70-Y493 antibody at 1:1000 dilution.Jurkat cells were treated by Hydrogen Peroxide at 37℃ for 2 minutes after serum-starvation overnight.Jurkat cells were treated by Pervanadate at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.

Immunohistochemistry of paraffin-embedded human breast carcinoma tissue, using Phospho-ZAP70-Y493 antibody .Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunofluorescence analysis of Jurkat cells using Phospho-ZAP70-Y493 Rabbit pAb at dilution of 1:100 .Jurkat cells were treated by Hydrogen Peroxide at 37℃ for 2 minutes after serum-starvation overnight. Blue: DAPI for nuclear staining.