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Phospho-eNOS-S1177 polyclonal antibody

货号: BS79371

价格：￥ 1680

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS79371

Product Name :Phospho-eNOS-S1177 polyclonal antibody

Swiss-Prot :P29474

Host :Rabbit

Reactivity :Human

Applications :WB

Application_all :WB,1:500 - 1:1000

Background :Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Phosphorylated

BiowMW :133kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :NOS3;ECNOS;eNOS

Immunogen :A synthetic phosphorylated peptide around S1177 of human eNOS(NP_000594.2).

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot analysis of extracts of Jurkat cells, using Phospho-eNOS-S1177 antibody .
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.

Western blot analysis of extracts of HeLa cells, using Phospho-NOS3-S1177 pAb at 1:1000 dilution or eNOS antibody .HeLa cells were treated by IGF-1 at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit .
Exposure time: 3min.