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Phospho-VEGF Receptor 2-Y1175 polyclonal antibody

货号: BS79370

价格：￥ 1680

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS79370

Product Name :Phospho-VEGF Receptor 2-Y1175 polyclonal antibody

Swiss-Prot :P35968

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB

Application_all :WB,1:500 - 1:1000

Background :Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Phosphorylated

BiowMW :230KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CD309;FLK1;VEGFR;VEGFR2;VEGF Receptor 2;KDR

Immunogen :A synthetic phosphorylated peptide around Y1175 of human VEGF Receptor 2(NP_002244.1).

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot analysis of A-431, using Phospho-VEGF Receptor 2-Y1175 antibody at 1:500 dilution.A-431 cells were treated by EGF at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.