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Phospho-CDK2-T160 polyclonal antibody

货号: BS79368

价格：￥ 1680

货期：现货（1-2个工作日）

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No. :BS79368

Product Name :Phospho-CDK2-T160 polyclonal antibody

Swiss-Prot :P24941

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:100

Background :This gene encodes a member of a family of serine/threonine protein kinases that participate in cell cycle regulation. The encoded protein is the catalytic subunit of the cyclin-dependent protein kinase complex, which regulates progression through the cell cycle. Activity of this protein is especially critical during the G1 to S phase transition. This protein associates with and regulated by other subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A), and p27Kip1 (CDKN1B). Alternative splicing results in multiple transcript variants.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Phosphorylated

BiowMW :42KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CDK2;CDKN2;p33(CDK2)

Immunogen :A synthetic phosphorylated peptide around T160 of human CDK2(NP_001789.2?/NP_001249.1).

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot analysis of C6, using Phospho-CDK2-T160 antibody at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human breast carcinoma using Phospho-CDK2-T160 antibody .Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.