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Phospho-EGFR-Y1068 polyclonal antibody

货号: BS79365

价格：￥ 1680

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

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说明书 MSDS SHIP

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No. :BS79365

Product Name :Phospho-EGFR-Y1068 polyclonal antibody

Swiss-Prot :P00533

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB, IP

Application_all :WB,1:1000 - 1:2000|IP,1:50 - 1:100

Background :The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Phosphorylated

BiowMW :185kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :EGFR;ERBB;ERBB1;HER1;NISBD2;PIG61;mENA

Immunogen :A synthetic phosphorylated peptide around Y1068 of human EGFR(NP_005219.2).

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot analysis of extracts of HeLa cells, using Phospho-EGFR-Y1068 pAb at 1:1000 dilution or EGFR antibody .HeLa cells were treated by EGF at 37℃ for 30 minutes after serum-starvation overnight.Hela cells were treated by ATP at 30℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit .
Exposure time: 1s.

Immunoprecipitation analysis of 200ug extracts of A-431 cells, using 3 ug Phospho-EGFR-Y1068 pAb . Western blot was performed from the immunoprecipitate using Phospho-EGFR-Y1068 pAb at a dilution of 1:1000. A-431 cells were treated by EGF at 37℃ for 30 minutes after serum-starvation overnight.