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Phospho-ERK1-T202 + ERK2-T185 polyclonal antibody

货号: BS79358

价格：￥ 1680

货期：现货（1-2个工作日）

25ul 50ul 100ul

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS79358

Product Name :Phospho-ERK1-T202 + ERK2-T185 polyclonal antibody

Swiss-Prot :P28482/P27361

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:100|IF/ICC,1:20 - 1:100

Background :The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Phosphorylated

BiowMW :42KDa/44KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :HMGCR;LDLCQ3;HMGCR

Immunogen :A synthetic phosphorylated peptide around T202 of human ERK1/ERK2(NP_002737.2).

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot analysis of extracts of various cell lines, using Phospho-ERK1-T202 antibody at 1:1000 dilution.Jurkat and NIH/3T3 cells were treated by PMA/TPA at 37℃ for 30 minutes after serum-starvation overnight.Jurkat cells were treated by ATP at 30℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.

Immunohistochemistry of paraffin-embedded human breast carcinoma tissue, using Phospho-ERK1-T202 antibody .Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunofluorescence analysis of methanol-fixed HeLa cells showing cytoplasmic, nuclear staining using Phospho-ERK1-T202 antibody .