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Phospho-Src-Y529 polyclonal antibody

货号: BS79356

价格：￥ 1680

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :BS79356

Product Name :Phospho-Src-Y529 polyclonal antibody

Swiss-Prot :P12931

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:100|IF/ICC,1:100 - 1:200

Background :This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Phosphorylated

BiowMW :60KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :ASV;SRC1;THC6;c-SRC;p60-Src;SRC

Immunogen :A synthetic phosphorylated peptide around Y529 of human Src(NP_005408.1).

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot analysis of extracts of A-431 cells, using Phospho-Src-Y529 antibody at 1:1000 dilution.A-431 cells were treated by EGF at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 60s.