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Phospho-FoxO1-S256 polyclonal antibody

货号: BS79355

价格：￥ 1680

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS79355

Product Name :Phospho-FoxO1-S256 polyclonal antibody

Swiss-Prot :Q12778

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:1000|IF/ICC,1:50 - 1:200

Background :This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Phosphorylated

BiowMW :100kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :FKH1;FKHR;FOXO1A;FOXO1

Immunogen :A synthetic phosphorylated peptide around S256 of human FoxO1(NP_002006.2).

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot analysis of extracts of 293T cells, using Phospho-FoxO1-S256 antibody .293T were treated by CIP at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit .
Exposure time: 90s.

Western blot analysis of extracts of HeLa cells, using Phospho-FoxO1-S256 pAb at 1:1000 dilution or FOXO1 antibody .Hela cell lysate were treated by CIP at 37℃ for 1 hour or treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight..
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit .
Exposure time: 2s.

Immunofluorescence analysis of NIH/3T3 using Phospho-FoxO1-S256 antibody at dilution of 1:50. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 using Phospho-FoxO1-S256 antibody at dilution of 1:50. Blue: DAPI for nuclear staining.